Product Description
Methylene Blue Hydrate from Loba India is a microscopy-grade biological stain supplied in a 25gm pack for supervised educational, analytical, research and professional laboratory work. The matched product is Loba Article No. 04645, grade For Microscopy, with packing code 00025 and Colour Index Number C.I. 52015.
Loba specifies a minimum dye content of 82%. The product is described as dark blue crystalline powder with a copper-coloured sheen, or green crystals with a bronze-coloured sheen. It is soluble in water and has a manufacturer-specified absorption maximum of 660-665nm in 50% ethanol.
The manufacturer states that Methylene Blue is used as a dye in several staining procedures, including Wright’s stain and Jenner’s stain. Laboratories may also use it in other approved microscopy, microbiology, histology or teaching methods where the procedure specifically calls for microscopy-grade methylene blue.
The current Loba SDS classifies the product as harmful if swallowed. The powder should be handled by trained personnel with suitable eye protection, gloves, protective clothing, dust control and secure storage. It can produce strong blue staining on skin, clothing, laboratory surfaces and porous materials.
This laboratory product is not intended for ingestion, self-medication, household treatment, food use, aquarium treatment or unsupervised student handling. Contact Inaya Scientific to confirm current stock, Bangladesh price, article number, batch COA, current SDS, expiry date and suitability for the intended staining or microscopy procedure before ordering.
Key Features
- Loba Chemie Article No. 04645.
- 25gm packing code: 00025.
- Grade: For Microscopy.
- Colour Index Number: C.I. 52015.
- Minimum dye content: 82%.
- Hydrate CAS number: 122965-43-9.
- EC number: 200-515-2.
- Molecular formula: C16H18ClN3S x H2O.
- Molecular weight: 319.859g/mol on an anhydrous basis.
- Dark blue crystalline powder with a copper-coloured sheen, or green crystals with a bronze-coloured sheen.
- Soluble in water and odourless.
- Manufacturer-listed melting point: approximately 190 degrees C.
- Absorption maximum: 660-665nm in 50% ethanol.
- Specific absorptivity A1%/1cm: 2250-2750 under the manufacturer-listed test conditions.
- Loss on drying at 105 degrees C: 8.0-22.0%.
- Suitable for approved microscopy staining procedures, including methods associated with Wright’s and Jenner’s stains.
- Listed shelf life: 60 months under recommended conditions.
- S. Code: 3204.1351.
- H302 – Harmful if swallowed.
- Not regulated as dangerous goods for road, sea or air transport under the available Loba SDS.
- Nationwide delivery may be available across Bangladesh, subject to stock and delivery confirmation.
Product Overview
Methylene Blue is a basic thiazine dye widely recognised for its intense blue colour and ability to bind to selected cellular and chemical structures. In microscopy, its colouring properties can improve contrast and make certain cells, microorganisms or structural details easier to observe when the procedure is suitable.
The matched Loba product is supplied as Methylene Blue Hydrate for Microscopy. The hydrate designation is important because the material contains variable water of hydration. The manufacturer therefore expresses the molecular weight on an anhydrous basis and includes a loss-on-drying specification of 8.0-22.0%.
Loba specifies minimum dye content rather than describing the material as a pure single-compound reagent. The 82% minimum dye content, spectral range and specific absorptivity form part of the product-performance specification. For critical staining, identity or absorbance work, the current batch COA should be reviewed.
Methylene Blue powder is normally used to prepare a solution at the concentration and solvent composition required by a recognised laboratory method. Different procedures may require different concentrations, buffers, pH conditions, filtration, ageing or combination with other dyes. Users should not prepare a general solution and assume it is suitable for every staining method.
Because Methylene Blue is also discussed in medical, industrial and consumer contexts, buyers should distinguish this microscopy-grade laboratory powder from pharmaceutical preparations, certified diagnostic kits, aquarium products and ready-made staining solutions. Grade, concentration, formulation and regulatory suitability are not interchangeable.
Main Applications
Microscopy Staining
Methylene Blue may be used in validated microscopy methods to increase contrast in selected cells, microorganisms or tissue structures. The concentration, staining time, rinse method and interpretation criteria should follow the approved protocol.
Wright’s and Jenner’s Stain Procedures
Loba specifically notes that Methylene Blue is used in staining procedures such as Wright’s stain and Jenner’s stain. These are formulated staining systems, so users must follow the complete recognised preparation and quality-control method rather than adding Methylene Blue without validation.
Simple Microbiological Staining
In supervised microbiology laboratories, Methylene Blue may be used in suitable simple-staining procedures to demonstrate microbial cell shape, size and arrangement. Smear preparation, heat fixing, biosafety and decontamination must follow the institutional SOP.
Educational Cell-Staining Demonstrations
Schools, colleges and universities may use dilute, method-approved Methylene Blue solutions in demonstrations involving selected plant, animal or microbial cells. Students should work under direct supervision with appropriate PPE and controlled quantities.
Preparation of Laboratory Stain Solutions
Trained laboratory personnel may prepare stain solutions from the powder using an approved formula. The finished solution should be labelled with its name, concentration, solvent, preparation date, expiry or review date, hazards and responsible person.
Spectrophotometric Teaching and Dye Studies
University laboratories may use Methylene Blue in selected absorbance, dilution, calibration or dye-behaviour experiments. Loba lists an absorption maximum of 660-665nm in 50% ethanol, but instrument settings and solvent conditions must match the method.
Redox Demonstrations
Methylene Blue may be included in selected supervised redox demonstrations because its colour can change with oxidation state. The reagents, concentrations and waste route must be selected through a recognised educational protocol.
Research and Quality-Control Work
The microscopy-grade dye may be used in validated research or quality-control methods when its minimum dye content, spectral specification and hydration range meet the procedure. Review the current batch COA for critical work.
Key Benefits
- Defined microscopy grade: The product is identified by Loba as For Microscopy.
- Minimum 82% dye content: The manufacturer provides a clear lower limit for dye content.
- Specified spectral performance: The absorption maximum and specific absorptivity support method verification.
- Strong visible colour: Small quantities can provide clear contrast in suitable staining procedures.
- Water-soluble powder: It can be prepared into method-specific aqueous solutions when the procedure allows.
- Broad educational value: It supports teaching in microscopy, microbiology, staining chemistry, dilution and spectrophotometry.
- Useful institutional pack: The 25gm size is practical for laboratories because many stain preparations require only small amounts.
- Verified manufacturer identity: Loba publishes the article number, grade, pack codes, dye content, physical properties and shelf life.
Who Should Buy This Product?
This product is suitable for properly equipped school chemistry and biology laboratories, college science departments, university laboratories, microbiology laboratories, medical laboratory training facilities, research institutes, quality-control departments and institutional procurement teams that require microscopy-grade Methylene Blue powder.
It may also suit trained teachers, lecturers, laboratory technicians, microbiologists, researchers and analysts who need a defined biological stain for an approved procedure or controlled laboratory demonstration.
This product is not intended for household colouring, food use, cosmetic use, aquarium treatment, self-medication, medical consumption or unsupervised experiments. Any clinical or diagnostic use requires a validated method, trained personnel, appropriate controls and compliance with applicable regulations.
Usage and Handling Summary
Methylene Blue should be handled only by trained personnel according to the supplied label, current Loba Safety Data Sheet, institutional chemical-hygiene plan and approved laboratory procedure.
Wear safety glasses or goggles, protective gloves and a laboratory coat. Minimise dust during weighing and transfer. Use good laboratory ventilation and appropriate local dust control when airborne powder may be generated.
Use clean, dry tools and containers. Weigh only the amount required by the method. Do not return unused powder to the original bottle because contamination or moisture uptake may affect dye performance.
Prepare stain solutions using the concentration, solvent, pH, filtration and storage conditions stated in the recognised method. Label every prepared solution with the product name, concentration, solvent, preparation date, expiry or review date, hazard information and responsible person.
For microscopy and microbiology procedures, use suitable positive and negative controls where required. Staining intensity can be influenced by concentration, staining time, pH, sample thickness, fixation, washing, lighting and microscope settings.
The current Loba SDS classifies Methylene Blue as harmful if swallowed. Do not eat, drink or smoke while using it. Wash hands, forearms and face thoroughly after handling, even when gloves were worn.
If swallowed, rinse the mouth and call a poison centre or doctor if the person feels unwell. For eye contact, rinse cautiously with water for several minutes and obtain medical advice if irritation continues. Wash exposed skin with plenty of water and remove contaminated clothing.
For a spill, keep unnecessary persons away and avoid creating dust. Mechanically recover the powder promptly with a scoop or suitable vacuum. Place recovered material and contaminated cleaning items in a labelled waste container for disposal at an authorised site.
Store the product in its original container in a cool, well-ventilated and secure place. Keep the container tightly closed and protect it from moisture, contamination, direct sunlight and unauthorised access.
Limitations and Important Considerations
This content applies specifically to Loba Methylene Blue Hydrate, Article No. 04645, grade For Microscopy, 25gm packing code 00025.
The product is specified at minimum 82% dye content and should not be described as 100% pure Methylene Blue.
The material is a hydrate, so water content can vary within the manufacturer-listed loss-on-drying range of 8.0-22.0%.
The current Loba SDS and product specification identify CAS 122965-43-9 for the hydrate form. CAS 61-73-4 may appear in references for anhydrous Methylene Blue; confirm the bottle label and COA.
The molecular weight of 319.859g/mol is stated on an anhydrous basis and should not be used as an exact hydrate formula weight without method-specific correction.
Staining performance depends on concentration, solvent, pH, staining time, fixation, washing, sample type and microscope conditions.
Methylene Blue alone is not equivalent to a prepared Wright’s stain, Jenner’s stain, Gram-stain kit or other formulated staining system.
The product should not automatically replace pharmaceutical-grade, diagnostic-grade, certified reference or aquarium-grade products.
Strongly coloured samples, over-staining, thick smears or poor washing may reduce contrast and create misleading observations.
The powder and prepared solutions can cause persistent blue staining of skin, clothing, benches, labels and porous laboratory materials.
This product is harmful if swallowed and must remain inaccessible to unauthorised persons and unsupervised students.
Prepared solutions may require shorter expiry periods than the original powder. Follow the preparation method and institutional stability policy.
The listed 60-month shelf life applies under manufacturer-specified conditions; confirm the actual expiry date and remaining shelf life on the supplied pack.
Do not use this laboratory reagent for ingestion, self-treatment, food colouring, household disinfection or aquarium treatment.
Clinical or diagnostic use requires validated procedures, quality controls, trained personnel and compliance with applicable regulations.
Dispose of unused dye, staining solutions, biological residues and contaminated packaging according to the applicable chemical and biological waste rules.
Contact Inaya Scientific for confirmation of the current batch COA, current SDS, dye content, expiry date, article number, stock status and method compatibility.
Available Pack Sizes or Variations
The confirmed product requested is:
25gm Methylene Blue Hydrate – Loba India, Article No. 04645, grade For Microscopy, packing code 00025
Loba also lists the following pack sizes for Article No. 04645:
- 100gm, packing code 00100
- 1000gm, packing code 01000
Availability may differ according to distributor stock, import schedule and current batch supply. Buyers should confirm the exact article number, grade, pack size, hydrate designation and bottle label before ordering.
Loba also supplies other Methylene Blue products, including AR-grade hydrate and prepared aqueous or microscopy solutions under different article numbers. These products can have different grades, concentrations, specifications and hazards and should not be treated as equivalent.
Methylene Blue Price in Bangladesh
The current Methylene Blue Loba India 25gm price in Bangladesh is available on request. The final price may vary according to article number, grade, batch, import cost, currency movement, stock status, freight, tax and delivery location.
Contact Inaya Scientific for the latest retail price, institutional quotation or bulk-order rate. Mention Loba Article No. 04645, grade For Microscopy and the 25gm pack when requesting a quotation to avoid confusion with AR-grade powder or prepared Methylene Blue solutions.
Availability and Delivery in Bangladesh
Methylene Blue Loba India 25gm is available from Inaya Scientific subject to current stock confirmation. Nationwide delivery support may be available across Bangladesh.
Delivery method, cost and destination coverage may depend on the quantity, packaging, carrier requirements and local transport conditions. Buyers should provide their organisation name, delivery location, required quantity and intended laboratory application when requesting an order confirmation.
Why Buy From Inaya Scientific?
Inaya Scientific supplies laboratory chemicals, educational laboratory items, instruments, glassware and scientific products to professional and institutional customers in Bangladesh. Buyers can verify important product and safety details before placing an order.
- Current stock and Bangladesh price confirmation.
- Brand, article number, grade and pack-size verification.
- Batch COA and current SDS availability confirmation.
- Dye-content, hydrate status and intended-application enquiry support.
- Bulk and institutional quotations.
- School, college, university, medical, research and quality-control laboratory sourcing support.
- Nationwide delivery coordination, subject to stock and carrier requirements.
Frequently Asked Questions
- What is Methylene Blue used for?
Methylene Blue is used as a biological dye in approved microscopy and staining procedures. Loba specifically identifies uses in staining methods such as Wright’s stain and Jenner’s stain.
- What is the Loba article number and grade?
The matched product is Loba Methylene Blue Hydrate, Article No. 04645, grade For Microscopy.
- What is the dye content of this product?
Loba specifies a minimum dye content of 82%. Review the current batch COA when dye performance is critical to the method.
- What is the CAS number of Loba Methylene Blue Hydrate?
The current Loba SDS identifies CAS 122965-43-9 for the hydrate product. Some references use CAS 61-73-4 for anhydrous Methylene Blue, so the supplied label and COA should be checked.
- Is Methylene Blue soluble in water?
Yes. Loba describes the product as soluble in water. The exact concentration and solvent conditions should follow the approved stain-preparation method.
- Can this powder be used directly on a microscope slide?
Usually, a method-specific staining solution is prepared first. Do not apply dry powder directly unless an approved procedure specifically requires it.
- Is Methylene Blue hazardous?
Yes. The current Loba SDS classifies it as harmful if swallowed, Acute Toxicity Category 4. Use gloves, eye protection, protective clothing and dust-control measures.
- How should Methylene Blue be stored?
Store it in the original tightly closed container in a cool, well-ventilated and secure place. Protect it from moisture, contamination, direct sunlight and unauthorised access.
- What is the price of Methylene Blue Loba 25gm in Bangladesh?
The current Bangladesh price is available from Inaya Scientific on request. Price may change with stock, import cost, exchange rate, freight, tax and batch availability.
- Can I order Methylene Blue with nationwide delivery?
Yes, subject to stock and delivery confirmation. Provide the required quantity, institution name, delivery location and intended laboratory use when requesting a quotation.
Order From Inaya Scientific
Order Methylene Blue Hydrate Loba India 25gm from Inaya Scientific for supervised school, college, university, microbiology, research and approved professional laboratory applications.
Seller: Inaya Scientific
Mobile: +880 1778-492798
WhatsApp: 01620247815
Email: inayascientific@gmail.com
Website: www.inayascientific.com
Delivery: Nationwide delivery across Bangladesh, subject to stock and carrier requirements
Before ordering, contact Inaya Scientific to confirm the current price, stock, exact brand, article number, grade, pack size, batch documents, expiry date and delivery conditions.
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