Product Description
Doxycycline DO 30 µg is a HiMedia antibiotic sensitivity disc intended for in vitro antimicrobial susceptibility testing of bacterial cultures by the Kirby-Bauer agar disc diffusion method. Each filter-paper disc contains 30 µg of doxycycline hydrochloride.
HiMedia supplies this product under catalogue number SD012. Each disc has a diameter of 6 mm and is printed with the identification code “DO 30” on the centre of both sides. This marking helps laboratory personnel identify the antimicrobial agent and potency during disc placement, incubation, zone measurement and result recording.
During testing, a standardised bacterial inoculum is spread uniformly over an appropriate agar medium. The Doxycycline DO 30 µg disc is placed aseptically on the inoculated surface using sterile forceps or a compatible disc dispenser. After incubation under the required conditions, the diameter of the complete inhibition zone is measured.
Zone measurements must be interpreted using the current CLSI, EUCAST or another approved standard applicable to the organism and laboratory programme. Breakpoints and quality-control ranges can change, so outdated criteria should not be used automatically.
Key Features
- Contains doxycycline hydrochloride at a potency of 30 µg per disc
- HiMedia catalogue number SD012
- Standard disc symbol DO
- Printed with DO 30 on both sides for easy identification
- 6 mm filter-paper disc format
- Designed for Kirby-Bauer agar disc diffusion testing
- Suitable for compatible bacterial cultures and approved susceptibility-testing methods
- Can be applied using sterile forceps or a compatible disc dispenser
- Convenient pack containing 50 discs
- Manufactured by HiMedia in India
- Suitable for professional microbiology and research laboratories
Product Overview
Antibiotic sensitivity discs are small standardised filter-paper discs impregnated with a defined amount of an antimicrobial agent. When placed on an inoculated agar plate, the agent diffuses into the surrounding medium and may inhibit the growth of a susceptible organism, producing a measurable zone around the disc.
Doxycycline is a tetracycline-class antimicrobial agent. In this HiMedia product, each disc contains 30 µg of doxycycline hydrochloride. The product is a microbiology testing reagent and is not a tablet, capsule, injection or other medicinal dosage form.
The inhibition-zone diameter can be influenced by the organism, agar medium, agar depth, pH, inoculum density, disc potency, storage history, incubation atmosphere, incubation duration and measurement technique. Reliable results therefore require a standardised procedure and acceptable quality-control performance.
General Specifications
Product Name: Doxycycline DO 30 µg
Official Chemical Name: Doxycycline Hydrochloride
Disc Symbol: DO
Potency: 30 µg per disc
Catalogue Number: SD012
Product Form: Antibiotic-impregnated filter-paper discs
Disc Diameter: 6 mm
Disc Marking: DO 30 printed on both sides
Brand: HiMedia
Country of Origin: India
Pack Size: 50 discs per pack
Packaging Format: Contact Inaya Scientific for confirmation
Recommended Test Method: Kirby-Bauer agar disc diffusion method
Recommended Medium: Appropriate susceptibility-testing medium selected for the organism
Storage Temperature: -20°C to +8°C
Storage Condition: Dry conditions with the supplied desiccant
Shelf Life: Use before the expiry date printed on the product label
Certificate of Analysis: Subject to batch and manufacturer availability
Technical Data Sheet: Available from HiMedia
Intended Use: Laboratory, research or further manufacturing use only
Main Applications
Antimicrobial Susceptibility Testing
Used to evaluate the in vitro response of compatible bacterial isolates to doxycycline by a standardised agar disc diffusion procedure.
Clinical Microbiology Laboratory Work
May be used in professional clinical microbiology workflows when the laboratory follows the applicable validated method, current interpretation standard, quality-control programme and regulatory requirements.
Pharmaceutical Microbiology
Suitable for laboratory research, quality-control studies, method verification and antimicrobial evaluation in pharmaceutical microbiology environments.
Public Health and Surveillance Testing
Can support approved antimicrobial-resistance surveillance programmes when used with appropriate organisms, culture media, reference strains and reporting procedures.
Research and Development
Useful for studies involving bacterial antimicrobial response, resistance patterns, comparative antimicrobial testing and disc diffusion performance.
Educational Microbiology
Suitable for supervised teaching and demonstration of agar diffusion principles in universities, medical colleges and laboratory training centres.
Food, Water and Veterinary Microbiology
May be used for research or surveillance testing of appropriate bacterial isolates when supported by a recognised method and laboratory quality system.
Key Benefits
- Standard 30 µg doxycycline potency for applicable susceptibility testing
- Clear DO 30 marking on both sides
- Compact 6 mm disc format
- Supports routine Kirby-Bauer disc diffusion workflows
- Pack of 50 discs is suitable for regular laboratory use
- No preparation of doxycycline solution is required before disc placement
- Compatible with sterile-forceps application and suitable disc dispensers
- Supported by HiMedia technical product documentation
- Easy identification through product code SD012
Who Should Buy This Product?
- Clinical microbiology laboratories
- Hospital and diagnostic laboratories
- Pharmaceutical quality-control laboratories
- Universities and medical colleges
- Research institutes
- Public health and antimicrobial-resistance surveillance laboratories
- Food microbiology laboratories
- Water-testing laboratories
- Veterinary microbiology laboratories
- Laboratory training centres
- Scientific and microbiology product suppliers
Basic Test Procedure
The following information is a general workflow only. The current approved method, HiMedia product instructions and the laboratory’s standard operating procedure must be followed.
- Prepare an appropriate susceptibility-testing agar plate with the method-specified depth, commonly about 4 mm for Mueller Hinton Agar.
- Prepare the test organism inoculum to the required density, commonly equivalent to a 0.5 McFarland standard when specified.
- Inoculate the complete agar surface uniformly with a sterile swab.
- Allow the inoculated surface to absorb excess moisture for the required period.
- Place the Doxycycline DO 30 µg disc aseptically using sterile forceps or a compatible disc dispenser.
- Maintain the required spacing between adjacent antimicrobial discs.
- Incubate at the temperature, atmosphere and duration specified for the test organism.
- Measure complete inhibition-zone diameters with a suitable calibrated measuring device.
- Interpret and report the result using the current approved breakpoint and quality-control standard.
Recommended Media and Testing Conditions
HiMedia identifies Mueller Hinton Agar for rapidly growing aerobic organisms in the Kirby-Bauer method. The medium should be prepared at the required depth and used with a standardised inoculum.
Selected fastidious organisms may require specialised or supplemented media, such as Haemophilus test medium, blood-supplemented Mueller Hinton Agar or suitable GC-based medium. The exact conditions must match the organism and current approved method.
The medium, inoculum density, disc spacing, incubation atmosphere, temperature, duration and interpretation criteria must be selected according to the organism. One set of conditions must not be applied to every bacterial species.
Storage and Shelf-Life
Store HiMedia Doxycycline SD012 discs at -20°C to +8°C under dry conditions with the desiccant supplied in the individual pack. Use the discs before the expiry date shown on the product label.
- Return the discs to the recommended storage condition promptly after use.
- Keep the vial, cartridge or package tightly closed.
- Protect the discs from moisture and prolonged room-temperature exposure.
- Do not use wet, damaged, contaminated or expired discs.
- Follow the laboratory procedure for temperature equilibration before opening cold packaging.
- Record the lot number, opening date, storage condition and expiry date.
- Do not use discs when required storage conditions have not been maintained.
Quality-Control Considerations
Each new lot or shipment should be evaluated through the laboratory’s quality-control programme using suitable reference organisms, approved culture media and standard incubation conditions.
Zone diameters may be affected by agar depth, medium pH, inoculum density, disc potency, storage conditions, incubation and measurement technique. Results should not be released when required quality-control performance is outside the accepted range.
Use the current CLSI, EUCAST or another approved quality-control and interpretation standard. Historical breakpoints or quality-control ranges should not be copied into routine use without confirming that they remain current and applicable.
Important Limitations
- Disc diffusion is an in vitro laboratory method and does not by itself predict the complete clinical outcome for a patient.
- The result depends on correct organism identification, inoculum preparation, medium selection, incubation and interpretation.
- This product must not be administered to humans or animals.
- The DO 30 disc must not be confused with doxycycline discs of another potency or product code.
- Susceptibility categories must be assigned using a current approved standard applicable to the organism.
- A small or absent inhibition zone should not be interpreted without confirming test validity and quality-control performance.
- The product is not intended for home testing or use by untrained individuals.
- A disc result should not replace authorised laboratory reporting or professional clinical judgement.
Safety and Handling
- Use only in a properly equipped microbiology laboratory.
- Handle bacterial cultures according to the required biosafety level and institutional procedure.
- Wear suitable gloves, laboratory clothing and eye protection.
- Apply discs using sterile forceps or a validated disc dispenser.
- Do not touch the discs with bare hands.
- Avoid unnecessary exposure of the discs to moisture and room air.
- Decontaminate used plates, cultures and disposable materials before disposal.
- Follow the product instructions, institutional biosafety procedure and applicable waste regulations.
Doxycycline DO 30 µg Price in Bangladesh
The price of HiMedia Doxycycline DO 30 µg discs in Bangladesh may depend on current stock, batch and expiry availability, import cost, controlled-storage requirements and order quantity.
Contact Inaya Scientific for the latest price, product-code confirmation, batch information, expiry date and delivery conditions.
Availability and Delivery in Bangladesh
HiMedia Doxycycline DO 30 µg antibiotic sensitivity discs, product code SD012, are available through Inaya Scientific subject to current stock and import availability.
Because antibiotic sensitivity discs require controlled storage, customers should confirm the delivery schedule, transportation arrangement and refrigerator or freezer storage preparation before ordering.
Nationwide delivery is available across Bangladesh.
Why Choose Inaya Scientific?
Inaya Scientific supplies microbiology products, culture media, antibiotic sensitivity discs, laboratory chemicals, scientific instruments and laboratory accessories to educational, research and industrial customers throughout Bangladesh.
Customers can contact Inaya Scientific to confirm:
- Current stock and batch number
- Expiry date
- Original HiMedia packaging
- Product code SD012
- Pack format and 50-disc quantity
- Storage and transportation arrangement
- Certificate of Analysis availability
- Technical Data Sheet availability
- Current price
- Institutional or bulk quotation
- Nationwide delivery arrangement
Frequently Asked Questions
What is Doxycycline DO 30 µg?
It is an antibiotic sensitivity test disc containing 30 µg of doxycycline hydrochloride for in vitro agar disc diffusion susceptibility testing.
What is the HiMedia product code?
The HiMedia catalogue number or SKU is SD012.
How many discs are supplied in one pack?
The specified package size is 50 discs per pack.
What is the diameter of each disc?
Each filter-paper disc has a diameter of 6 mm.
How is the disc identified?
Both sides of the disc are printed with DO 30 for identification of the antimicrobial agent and potency.
Which method is it used for?
It is intended for antimicrobial susceptibility testing by the Kirby-Bauer agar disc diffusion method or another approved standardised procedure.
Which medium should be used?
Mueller Hinton Agar is commonly used for rapidly growing aerobic bacteria. Selected fastidious organisms may require specialised or supplemented media.
How should the discs be stored?
Store the discs at -20°C to +8°C under dry conditions with the supplied desiccant and use them before the expiry date.
Can it be used as medicine?
No. It is a laboratory susceptibility-testing disc and must not be administered to humans or animals.
Can old breakpoint values be used?
No. Use current CLSI, EUCAST or another approved criterion because breakpoints and quality-control ranges may be revised.
Is a Certificate of Analysis available?
COA availability depends on the product batch and manufacturer system. Contact Inaya Scientific for confirmation.
Can it be delivered outside Dhaka?
Yes. Nationwide delivery is available across Bangladesh, subject to suitable controlled-storage and transportation arrangements.
Closing Recommendation
HiMedia Doxycycline DO 30 µg antibiotic sensitivity discs are suitable for professional laboratories requiring a standard 30 µg doxycycline disc for agar diffusion susceptibility testing. The 6 mm discs, DO 30 marking, SD012 product code and 50-disc pack make the product practical for routine microbiology work.
For dependable results, laboratories should maintain -20°C to +8°C dry storage, use appropriate culture media and quality-control organisms, follow a validated testing procedure and interpret inhibition zones using the current approved standard.
Order From Inaya Scientific
Seller: Inaya Scientific
Product: Doxycycline DO 30 µg
Official Product Name: Doxycycline Hydrochloride
Disc Symbol: DO
Disc Marking: DO 30
Brand: HiMedia
Product Code: SD012
Country of Origin: India
Potency: 30 µg per disc
Pack Size: 50 discs per pack
Mobile: +880 1778-492798
WhatsApp: 01620-247815
Email: inayascientific@gmail.com
Website: www.inayascientific.com
Delivery: Nationwide delivery across Bangladesh
When requesting a quotation, mention the product name, product code SD012, required quantity, delivery address and contact number.
